Synthesising Enzymes

Synthesising Enzymes-82
Geraniol esters were synthesised by direct esterification catalysed by esterases and lipases (five enzymes were tested) in a solvent-free system at 37°C.Our programs in this area include the development of novel substrates and the exploitation of native and modified enzymes for organic synthesis.Since these 1,3-polyol systems are useful synthons, we have further examined the scope of this enzymatic methodology.

Geraniol esters were synthesised by direct esterification catalysed by esterases and lipases (five enzymes were tested) in a solvent-free system at 37°C.

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Our synthetic strategy emphasizes a combination of chemical and enzymatic methods, with particular focus on the use of enzymes for stereocontrolled processes.

Major efforts in synthetic carbohydrate chemistry include asymmetric aldol condensations for the synthesis of novel monosaccharides based on aldolases, and stereocontrolled synthesis of oligosaccharides and glycopeptides based on glycosyl transferases.

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In a study co-led by a team of the Department of Inorganic and Organic Chemistry and the Institute of Theoretical and Computational Chemistry of the UB (IQTCUB), together with a research group of biological chemistry of the University of Oxford, have synthesized carbohydrates with enzymes through a reaction that was not much studied so far on these biomolecules and which creates few by-products.

, has been led by the researcher Carme Rovira, ICREA researcher from the IQTCUB, and Benjamin G. The study also has the participation of Javier Iglesias-Fernández, researcher in training at the UB and first author of the study in which the supercomputer Mare Nostrum (from Barcelona Supercomputing Center, BSC-CNS) was used to create multi-scale molecular dynamics simulations.New enzymes that go from degrading to synthesizing carbohydrates Now the teams from Barcelona and Oxford have found the way to modify an enzyme (glycosidase) which usually degrades carbohydrates (it catalyses the hydrolysis in glycosidic bonds) so that it acts reversely and synthesizes carbohydrates."Moreover, this synthesis has been reached with a new reaction that had not been observed in glycosidase yet.We report a systematic study of a modular approach to create multi-component supramolecular nanostructures that can be tailored to be both enzyme and temperature responsive.Using a straightforward synthetic approach we functionalised a thermal responsive polymer, poly(2-isopropyl-2-oxazoline), with fluorenylmethoxycarbonyl-amino acids that drive the self-assembly.The hemiacetal could be further oxidized to give a lactone which can be further transformed to other useful synthons. Please select whether you prefer to view the MDPI pages with a view tailored for mobile displays or to view the MDPI pages in the normal scrollable desktop version.Her team discovered the molecular bases of the one side mechanism in enzymes in 2001.In the study, the synthesis has been carried out with a specific enzyme, the Sulfolobus solfataricus beta-glycosidase, but it can be applied to other enzymes using a similar engineering method of the active center.This is a variation from a reaction called front-face or SNi-like" says Carme Rovira."The advantage of this reaction in glycosidase -continues the researcher- is that it makes the synthesis in a clean way, without almost causing hydrolysis residues and using economically viable substrates (sugar)." "Basically, we used our experience with enzymes that synthesize carbohydrates to apply the reaction to one side in other enzymes with more biotechnological possibilities" concluded the researcher.


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