Transgenic Animals Research Papers

The nuclear transfer application may be more useful and beneficial for agricultural is the ability to efficiently produce a large number of identical offspring derived from a particular mating.Therefore, nuclear transfer using a embryonic cell lines derived from that mating maybe more attractive Gametes are incubated during short time periods in a solution containing the gene constructions and then they are checked for transfection, used for inseminations or for in vitro fertilization procedures.

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This could happen if the gene inserts itself in an area of DNA that is not normally expressed, so a sample of the pups tissue from the tail will be taken and DNA examined.

There was generation of three healthy identical female offspring.

The application of transgenic animals showed that within the next five to eight years genetically modified animals will play a significant and important role in the biomedical field, in particular via the production of valuable pharmaceutical proteins and the supply of xenografts.

This paper attempts to give an idea about development of transgenic animals as well as their application for human welfare.

After using the in vitro or in vivo transfection procedures high percentages (80%) of spermatozoa appeared transfected.

These results usually showed that the foreign gene appeared into the nucleus of spermatozoa and molecular procedures (Slot-Blot, PCR, Southern Blot and gene sequences) have shown the presence of the transgene in the DNA of the gametes Also, Yeast Artificial Chromosomes (YACs) transgenic mice are generated by using pronuclear microinjection and represents latest generation of vectors which have the great advantage of large insert size. (A) A single-cell suspension is produced from a fertile donor testis.

There are several types of transgenic animals like transgenic sheep, birds, chickens, pigs, insects etc.

Some important benefits and risks of transgenic animals are shown in table 1.

Genotypic analyses confirmed that all cloned offspring were derived from the donor cell line.

Analysis of the milk of one of the transgenic cloned animals showed high-level production of human antithrombin III.


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